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List of restriction enzyme cutting sites: L–N

From Wikipedia, the free encyclopedia

Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T

This article contains a list of the most studied restriction enzymes whose names start with L to N inclusive. It contains approximately 120 enzymes.

The following information is given:

  • Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
  • Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme:  EcoR70I 


Whole list navigation

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Restriction enzymes

[edit]

L

[edit]
Enzyme PDB code Source Recognition sequence Cut Isoschizomers
LcaI Lactobacillus casei 5' ATCGAT
3' TAGCTA
5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'
BdiI, Bli41I, Bsa29I, BscI, Bsp106I, BspDI, Bst28I, LplI, PgaI, ZhoI
LlaAI Lactococcus lactis cremoris W9 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
Bme12I, Bsp67I, BstENII, ChaI, FnuEI, MgoI, NphI, RalF40I
LlaBI Lactococcus lactis cremoris W56 5' CTRYAG
3' GAYRTC
5' ---C   TRYAG--- 3'
3' ---GAYRT   C--- 5'
LlaCI Lactococcus lactis W15 5' AAGCTT
3' TTCGAA
5' ---A   AGCTT--- 3'
3' ---TTCGA   A--- 5'
LlaG2I Lactococcus lactis cremoris 5' GCTAGC
3' CGATCG
5' ---G   CTAGC--- 3'
3' ---CGATC   G--- 5'
AceII, AsuNHI, BmtI,  BspOI, 
NheI, PstNHI
Lmu60I Listeria murrayi 60 5' CCTNAGG
3' GGANTCC
5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5'
AocI, AxyI, BspR7I, CvnI, Lmu60I, MstII, SauI, SshAI
LplI Lactobacillus plantarum 5' ATCGAT
3' TAGCTA
5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'
BanIII, BbvAII, BdiI, Bsa29I, BscI, BstNZ169I, ClaI, LcaI, PgaI
LpnI Legionella pneumophila 5' RGCGCY
3' YCGCGR
5' ---RGC   GCY--- 3'
3' ---YCG   CGR--- 5'
AccB2I,  BfoI,  Bme142I,
Bsp143II, BstH2I, HaeII
LspI Lactobacillus sp. 5' TTCGAA
3' AAGCTT
5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5'
Asp10HI, BimI, BsiCI, BstBI, Csp45I, PlaII, PpaAI, Ssp1I
LweI Listeria welshimeri RFL131 5' GCATC
3' CGTAG
5' ---GCATCN4N   NNNN--- 3'
3' ---CGTAGN4NNNNN   --- 5'

M

[edit]
Enzyme PDB code Source Recognition sequence Cut Isoschizomers
MabI Microbacterium arborescens SE 5' ACCWGGT
3' TGGWCCA
5' ---A   CCWGGT--- 3'
3' ---TGGWCC   A--- 5'
MaeI Methanococcus aeolicus PL-15/H 5' CTAG
3' GATC
5' ---C   TAG--- 3'
3' ---GAT   C--- 5'
MaeII Methanococcus aeolicus 5' ACGT
3' TGCA
5' ---A   CGT--- 3'
3' ---TGC   A--- 5'
MaeIII Methanococcus aeolicus 5' GTNAC
3' CANTG
5' ---   GTNAC--- 3'
3' ---CANTG   --- 5'
MaeK81I Microcystis aeruginosa K-81 5' CGTACG
3' GCATGC
5' ---C   GTACG--- 3'
3' ---GCATG   C--- 5'
MaeK81II Microcystis aeruginosa 5' GGNCC
3' CCNGG
5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'
AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, Sau96I
MamI Microbacterium ammoniaphilum 5' GATN4ATC
3' CTAN4TAG
5' ---GATNN   NNATC--- 3'
3' ---CTANN   NNTAG--- 5'
MavI Mycobacterium avium 5' CTCGAG
3' GAGCTC
5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5'
BspAAI, BstVI, MlaAI, PaeR7I, SauLPII, SciI, SlaI, Sol10179I, XhoI
MbiI Moraxella bovis Fr1-022 5' CCGCTC
3' GGCGAG
5' ---CCG   CTC--- 3'
3' ---GGC   GAG--- 5'
AccBSI, BsrBI, BstD102I, Bst31NI
MboI Moraxella bovis 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
AspMDI, Bsp105I, BspFI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I
MboII Moraxella bovis 5' GAAGA
3' CTTCT
5' ---GAAGAN6NN   --- 3'
3' ---CTTCTN6N   N--- 5'
MchI Micromonospora chalcea 5' GGCGCC
3' CCGCGG
5' ---GG   CGCC--- 3'
3' ---CCGC   GG--- 5'
MchAI Mycobacterium chelonae 5' GCGGCCGC
3' CGCCGGCG
5' ---GC   GGCCGC--- 3'
3' ---CGCCGG   CG--- 5'
MchAII Mycobacterium chelonae 5' GGCC
3' CCGG
5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
McrI Micrococcus cryophilus 5' CGRYCG
3' GCYRGC
5' ---CGRY   CG--- 3'
3' ---GC   YRGC--- 5'
MfeI Mycoplasma fermentans 5' CAATTG
3' GTTAAC
5' ---C   AATTG--- 3'
3' ---GTTAA   C--- 5'
MflI Microbacterium flavum 5' RGATCY
3' YCTAGR
5' ---R   GATCY--- 3'
3' ---YCTAG   R--- 5'
MfoAI Mycobacterium fortuitum TMC 1529 5' GGCC
3' CCGG
5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
Mgl14481I Merismopedia glauca 1448-1 5' CCSGG
3' GGSCC
5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5'
AseII, AsuC2I, BcnI, BpuMI, CauII, Eco1831I, EcoHI, HgiS22I, NciI
MgoI Mycobacterium gordonae 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
AspMDI, BspAI, BstENII, ChaI, FnuEI, MkrAI, NphI, Sau3AI
MhaAI Mycobacterium habana 206 5' CTGCAG
3' GACGTC
5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5'
AliAJI, Asp713I, Bsp63I, CstI, Ecl37kI, HalII, PaePI, PstI, YenI
MhlI Micrococcus halobius SD 5' GDGCHC
3' CHCGDG
5' ---GDGCH   C--- 3'
3' ---C   HCGDG--- 5'
AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, SduI
MkrAI Micrococcus kristinae 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
Bme12I, BspAI, BstENII, ChaI, FnuEI, MgoI, NphI, Sau3AI
MlaI Mastigocladus laminosus 5' TTCGAA
3' AAGCTT
5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5'
Asp10HI, BimI, BsiCI, BstBI, Csp68KII, LspI, PlaII, Ssp1I
MlaAI Micromonospora lacustris 5' CTCGAG
3' GAGCTC
5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5'
BspAAI, BstVI, PanI, SauLPII, Sbi68I, Sfr274I, SlaI, XhoI
MlsI Micrococcus luteus Ng 16-122 5' TGGCCA
3' ACCGGT
5' ---TGG   CCA--- 3'
3' ---ACC   GGT--- 5'
MltI Micrococcus luteus 5' AGCT
3' TCGA
5' ---AG   CT--- 3'
3' ---TC   GA--- 5'
AluBI, AluI
MluI Micrococcus luteus 5' ACGCGT
3' TGCGCA
5' ---A   CGCGT--- 3'
3' ---TGCGC   A--- 5'
Mlu23I Micrococcus luteus 23 5' GGATCC
3' CCTAGG
5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5'
Mlu31I Micrococcus luteus 31 5' TGGCCA
3' ACCGGT
5' ---TGG   CCA--- 3'
3' ---ACC   GGT--- 5'
MluB2I Micrococcus luteus B2 5' TCGCGA
3' AGCGCT
5' ---TCG   CGA--- 3'
3' ---AGC   GCT--- 5'
MluNI Micrococcus luteus N 5' TGGCCA
3' ACCGGT
5' ---TGG   CCA--- 3'
3' ---ACC   GGT--- 5'
MlyI Micrococcus lylae 5' GAGTC
3' CTCAG
5' ---GAGTCN4N   --- 3'
3' ---CTCAGN4N   --- 5'
Mly113I Micrococcus lylae 113 5' GGCGCC
3' CCGCGG
5' ---GG   CGCC--- 3'
3' ---CCGC   GG--- 5'
MmeI Methylophilus methylotrophus 5' TCCRAC
3' AGGYTG
5' ---TCCRACN17NNN   --- 3'
3' ---AGGYTGN17N   NN--- 5'
MnlI Moraxella nonliquefaciens 5' CCTC
3' GGAG
5' ---CCTCN4NNN   --- 3'
3' ---GGAGN4NN   N--- 5'
MnoI Moraxella nonliquefaciens 5' CCGG
3' GGCC
5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
Mph1103I Moraxella phenylpyruvica RFL1103 5' ATGCAT
3' TACGTA
5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5'
BfrBI, Csp68KIII, EcoT22I, NsiI, PinBI, Ppu10I, SepI, SspD5II
MroI Micrococcus roseus 5' TCCGGA
3' AGGCCT
5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5'
BbvAIII, BseAI, Bsp13I, BspEI, Bsu23I, CauB3I, PinBII, PtaI
MroNI Micrococcus roseus N 5' GCCGGC
3' CGGCCG
5' ---G   CCGGC--- 3'
3' ---CGGCC   G--- 5'
MroXI Micrococcus roseus XM 5' GAAN4TTC
3' CTTN4AAG
5' ---GAANN   NNTTC--- 3'
3' ---CTTNN   NNAAG--- 5'
Asp700I, BbvAI, PdmI, XmnI
MscI Micrococcus sp. 5' TGGCCA
3' ACCGGT
5' ---TGG   CCA--- 3'
3' ---ACC   GGT--- 5'
MseI Micrococcus sp. 5' TTAA
3' AATT
5' ---T   TAA--- 3'
3' ---AAT   T--- 5'
MslI Moraxella osloensis 5' CAYN4RTG
3' GTRN4YAC
5' ---CAYNN   NNRTG--- 3'
3' ---GTRNN   NNYAC--- 5'
MspI 1SA3, 1YFI, Search PDBe Moraxella sp. 5' CCGG
3' GGCC
5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
Msp17I Micrococcus sp. 17 5' GRCGYC
3' CYGCRG
5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5'
AsuIII, BsaHI, BstACI, HgiGI, HgiHII, Hin1I, Msp17I, PamII
Msp20I Micrococcus sp. 5' TGGCCA
3' ACCGGT
5' ---TGG   CCA--- 3'
3' ---ACC   GGT--- 5'
Msp67I Micrococcus sp. MS67 5' CCNGG
3' GGNCC
5' ---CC   NGG--- 3'
3' ---GGN   CC--- 5'
MspA1I Moraxella sp. A1 5' CMGCKG
3' GKCGMC
5' ---CMG   CKG--- 3'
3' ---GKC   GMC--- 5'
MspB4I Moraxella sp. B4 5' GGYRCC
3' CCRYGG
5' ---G   GYRCC--- 3'
3' ---CCRYG   G--- 5'
AccB1I, BanI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, PfaAI
MspCI Micrococcus sp. 5' CTTAAG
3' GAATTC
5' ---C   TTAAG--- 3'
3' ---GAATT   C--- 5'
MspR9I Micrococcus sp. R9 5' CCNGG
3' GGNCC
5' ---CC   NGG--- 3'
3' ---GGN   CC--- 5'
MspSWI Moraxella sp. SW 5' ATTTAAAT
3' TAAATTTA
5' ---ATTT   AAAT--- 3'
3' ---TAAA   TTTA--- 5'
MspV281I Myxosarcinia sp. V/281 5' GWGCWC
3' CWCGWG
5' ---GWGCW   C--- 3'
3' ---C   WCGWG--- 5'
Alw21I, AspHI, Bbv12I, Bsh45I, BsiHKAI,
 Bsm6I,  HgiAI,  HpyF46II, 
MspYI Microcystis sp. J2 5' YACGTR
3' RTGCAY
5' ---YAC   GTR--- 3'
3' ---RTG   CAY--- 5'
MssI Methylobacterium sp. Dd 5-732 5' GTTTAAAC
3' CAAATTTG
5' ---GTTT   AAAC--- 3'
3' ---CAAA   TTTG--- 5'
PmeI
MstI Microcoleus sp. 5' TGCGCA
3' ACGCGT
5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5'
Acc16I, AosI, AviII, FdiII, FspI, NsbI, PamI, Pun14627I
MstII Microcoleus sp. 5' CCTNAGG
3' GGANTCC
5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5'
AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI
MthZI Methanobacterium thermoformicicum Z-245 5' CTAG
3' GATC
5' ---C   TAG--- 3'
3' ---GAT   C--- 5'
MunI 1D02 Mycoplasma sp. 5' CAATTG
3' GTTAAC
5' ---C   AATTG--- 3'
3' ---GTTAA   C--- 5'
MvaI 2OAA, 2OA9, Search PDBe, 2OA9 Micrococcus varians RFL19 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AjnI, BciBII, BptI, Bst1I, BstOI, BstM6I, Bst2UI, SslI, TaqXI, ZanI
Mva1269I Micrococcus varians RFL1269 5' GAATGC
3' CTTACG
5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5'
Asp26HI, Asp40HI, Asp50HI, BscCI, BsmI, Mva1269I, PctI
MvnI Methanococcus vannielii 5' CGCG
3' GCGC
5' ---CG   CG--- 3'
3' ---GC   GC--- 5'
AccII, Bsh1236I, BstFNI, BtkI, Csp68KVI, FalII, FauBII, ThaI
MvrI Micrococcus varians 5' CGATCG
3' GCTAGC
5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5'
Afa22MI, BspCI, EagBI, ErhB9I, Ple19I, PvuI, RshI, XorII
MwoI Methanobacterium wolfei 5' GCN7GC
3' CGN7CG
5' ---GCNNNNN   NNGC--- 3'
3' ---CGNN   NNNNNCG--- 5'
MxaI Myxococcus xanthus F18E 5' GAGCTC
3' CTCGAG
5' ---GAG   CTC--- 3'
3' ---CTC   GAG--- 5'

N

[edit]
Enzyme PDB code Source Recognition sequence Cut Isoschizomers
NaeI 1EV7, 1iaw, Search PDB Nocardia aerocolonigenes 5' GCCGGC
3' CGGCCG
5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
NarI Nocardia argentinensis 5' GGCGCC
3' CCGCGG
5' ---GG   CGCC--- 3'
3' ---CCGC   GG--- 5'
NblI Nocardia blackwellii 5' CGATCG
3' GCTAGC
5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5'
Afa16RI, Afa22MI, EagBI, MvrI, Ple19I, Psu161I, RshI, XorII
NciI Neisseria cinerea 5' CCSGG
3' GGSCC
5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5'
AhaI, AseII, AsuC2I,   BpuMI,   
CauII, EcoHI, HgiS22I, Mgl14481I
NcoI Nocardia corallina 5' CCATGG
3' GGTACC
5' ---C   CATGG--- 3'
3' ---GGTAC   C--- 5'
NcrI Nocardia carnea C-212 5' AGATCT
3' TCTAGA
5' ---A   GATCT--- 3'
3' ---TCTAG   A--- 5'
NcuI Neisseria cuniculi 5' GAAGA
3' CTTCT
5' ---GAAGAN6NN   --- 3'
3' ---CTTCTN6N   N--- 5'
NdaI Nocardia dassonvillei 5' GGCGCC
3' CCGCGG
5' ---GG   CGCC--- 3'
3' ---CCGC   GG--- 5'
NdeI Neisseria denitrificans 5' CATATG
3' GTATAC
5' ---CA   TATG--- 3'
3' ---GTAT   AC--- 5'
NdeII Neisseria denitrificans 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
Bce243I, Bsp105I, BspJI, BstMBI, CviAI, Kzo9I, NlaII, Sth368I
NgoAIII Neisseria gonorrhoeae FA1090 5' CCGCGG
3' GGCGCC
5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
NgoAIV Neisseria gonorrhoeae FA1090 5' GCCGGC
3' CGGCCG
5' ---G   CCGGC--- 3'
3' ---CGGCC   G--- 5'
NgoMIV 1FIU, 4abt Search PDBe θ Neisseria gonorrhoeae MS11 5' GCCGGC
3' CGGCCG
5' ---G   CCGGC--- 3'
3' ---CGGCC   G--- 5'
NgoPII Neisseria gonorrhoeae P9-2 5' GGCC
3' CCGG
5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
NgoPIII Neisseria gonorrhoeae P9-2 5' CCGCGG
3' GGCGCC
5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
NheI Neisseria mucosa heidelbergensis 5' GCTAGC
3' CGATCG
5' ---G   CTAGC--- 3'
3' ---CGATC   G--- 5'
AceII, AsuNHI, BmtI,  BspOI, 
LlaG2I, PstNHI
NlaII Neisseria lactamica 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
BscFI, BspAI, BstENII, ChaI, FnuEI, MgoI, RalF40I, Sau3AI
NlaIII Neisseria lactamica 5' CATG
3' GTAC
5' ---CATG   --- 3'
3' ---   GTAC--- 5'
NlaIV Neisseria lactamica 5' GGNNCC
3' CCNNGG
5' ---GGN   NCC--- 3'
3' ---CCN   NGG--- 5'
AspNI, BscBI,   BmiI,  BspLI,
PspN4I
Nli3877I Nostoc linckia 5' CYCGRG
3' GRGCYC
5' ---CYCGR   G--- 3'
3' ---G   RGCYC--- 5'
Ama87I, AvaI, BcoI, BsiHKCI, Eco27kI, Eco88I, NspSAI, OfoI
NmeCI Neisseria meningitidis C114 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
BscFI, BspAI, BstENII, ChaI, FnuEI, MgoI, RalF40I, Sau3AI
NmeRI Neisseria meningitidis 5' CAGCTG
3' GTCGAC
5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'
NmuCI Neisseria mucosa C9-2 5' GTSAC
3' CASTG
5' ---   GTSAC--- 3'
3' ---CASTG   --- 5'
NopI Nocardia opaca 5' GTCGAC
3' CAGCTG
5' ---G   TCGAC--- 3'
3' ---CAGCT   G--- 5'
NotI 3C25, 3BVQ, Search PDBe Nocardia otitidis-caviarum 5' GCGGCCGC
3' CGCCGGCG
5' ---GC   GGCCGC--- 3'
3' ---CGCCGG   CG--- 5'
NphI Neisseria pharyngis C245 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
AspMDI, BspAI, BstENII, ChaI, FnuEI, MkrAI, RalF40I, Sau3AI
NruI Nocardia rubra 5' TCGCGA
3' AGCGCT
5' ---TCG   CGA--- 3'
3' ---AGC   GCT--- 5'
NruGI Nocardia sp. G 5' GACN5GTC
3' CTGN5CAG
5' ---GACNNN   NNGTC--- 3'
3' ---CTGNN   NNNCAG--- 5'
AhdI, AspEI,  BmeRI,  BspOVI,
DriI, Eam1105I, EclHKI
NsbI Neisseria subflava Va-1 5' TGCGCA
3' ACGCGT
5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5'
Acc16I, AosI, AviII, FdiII, FspI, MstI, PamI, Pun14627I
NsiI Neisseria sicca 5' ATGCAT
3' TACGTA
5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5'
BfrBI, Csp68KIII, EcoT22I, PinBI, Ppu10I, SepI, SspD5II, Zsp2I
NsiCI Neisseria sicca C351 5' GATATC
3' CTATAG
5' ---GAT   ATC--- 3'
3' ---CTA   TAG--- 5'
NspI Nostoc sp. C 5' RCATGY
3' YGTACR
5' ---RCATG   Y--- 3'
3' ---Y   GTACR--- 5'
NspII Nostoc sp. C 5' GDGCHC
3' CHCGDG
5' ---GDGCH   C--- 3'
3' ---C   HCGDG--- 5'
AocII, BmyI, BsoCI, Bsp1286I, MhlI, NspII, SduI
NspIII Nostoc sp. C 5' CYCGRG
3' GRGCYC
5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5'
Ama87I, AvaI, Bse15I, BsoBI, Eco27kI, Eco88I, OfoI, PunAI
NspIV Nostoc sp. C 5' GGNCC
3' CCNGG
5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'
AvcI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, UnbI
NspV Nostoc sp. C 5' TTCGAA
3' AAGCTT
5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5'
Asp10HI, BimI, Bsp119I, BstBI, Csp68KII, MlaI, PlaII, SspRFI
Nsp7121I Nostoc sp. 7121 5' GGNCC
3' CCNGG
5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'
AvcI, Bal228I, Bce22I, Bsp1894I, Bsu54I, Cfr13I, NspIV, UnbI
Nsp29132II Nostoc sp. 5' GGATCC
3' CCTAGG
5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5'
AliI, ApaCI, BamHI, Bce751I, Nsp29132II, Pfl8I, SolI, Uba4009I
NspBII Nostoc sp. 5' CMGCKG
3' GKCGMC
5' ---CMG   CKG--- 3'
3' ---GKC   GMC--- 5'
NspHI Nostoc sp. 5' RCATGY
3' YGTACR
5' ---RCATG   Y--- 3'
3' ---Y   GTACR--- 5'
NspLKI Nocardia sp. LK 5' GGCC
3' CCGG
5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
NspMACI Nostoc sp. 5' AGATCT
3' TCTAGA
5' ---A   GATCT--- 3'
3' ---TCTAG   A--- 5'
NspSAI Nostoc sp. SA 5' CYCGRG
3' GRGCYC
5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5'
Ama87I, AvaI, Bse15I, BspLU4I, Eco27kI, Nli3877I, OfoI, PunAI
NspSAII Nostoc sp. SA 5' GGTNACC
3' CCANTGG
5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5'
AspAI, Bse64I, BseT9I, BseT10I, BstPI, Eco91I, NspSAII, PspEI
NspSAIV Nostoc sp. SA 5' GGATCC
3' CCTAGG
5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5'
AliI, BamHI, Bsp98I, Bsp4009I, NspSAIV, Pfl8I, SolI, Uba4009I
NunII Nocardia uniformis 5' GGCGCC
3' CCGCGG
5' ---GG   CGCC--- 3'
3' ---CCGC   GG--- 5'

Notes

[edit]
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  3. ^ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
  4. ^ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
  5. ^ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
  6. ^ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.