Cas1
| CRISPR-associated protein 1 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
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| Identifiers | |||||||||
| Symbol | cas1 | ||||||||
| Pfam | PF01867 | ||||||||
| InterPro | IPR002729 | ||||||||
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CRISPR-associated protein 1 (cas1) is one of the two universally conserved proteins found in the CRISPR prokaryotic immune defense system. Cas1 is a metal-dependent DNA-specific endonuclease that produces double-stranded DNA fragments.[1] Cas1 forms a stable complex with the other universally conserved CRISPR-associated protein, cas2, which is essential to spacer acquisition for CRISPR systems.[2]
In July 2017, researchers led by Jennifer Doudna from the University of California at Berkeley, in Berkeley, California, using electron microscopy and X-ray crystallography, at the Advanced Light Source at Lawrence Berkeley National Laboratory, the Stanford Linear Accelerator Center, and the HHMI electron microscope facility at UC Berkeley, discovered how Cas1-Cas2, the proteins responsible for the ability of the CRISPR immune system (CRISPR means: clustered regularly interspaced short palindromic repeats) in bacteria to adapt to new viral infections, identify the site in the genome where they insert viral DNA so they can recognize it later and mount an attack. A protein called IHF plays a crucial role in this process.[3] Scientists also discovered that Cas-1 inhibits Cas-2/3 enzymatic activity as a nuclease and in the same discussion postulated that Cas1-Cas2 had an evolutionary origin as a toxin-antitoxin complex. This could result in a change in the evolutionary model of the Cas1-Cas2 complex.[4]
References
[edit]- ^ Wiedenheft B, Zhou K, Jinek M, Coyle SM, Ma W, Doudna JA (2009). "Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated genome defense". Structure. 17 (6): 904–12. doi:10.1016/j.str.2009.03.019. PMID 19523907.
- ^ Nuñez JK, Kranzusch PJ, Noeske J, Wright AV, Davies CW, Doudna JA (2014). "Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity". Nat. Struct. Mol. Biol. 21 (6): 528–34. doi:10.1038/nsmb.2820. PMC 4075942. PMID 24793649.
- ^ "Researchers discover how CRISPR proteins find their target". 20 July 2017.
- ^ Rollins, MaryClare F.; Chowdhury, Saikat; Carter, Joshua; Golden, Sarah M.; Wilkinson, Royce A.; Bondy-Denomy, Joseph; Lander, Gabriel C.; Wiedenheft, Blake (24 April 2017). "Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity". Proceedings of the National Academy of Sciences. 114 (26): E5113–E5121. Bibcode:2017PNAS..114E5113R. doi:10.1073/pnas.1616395114. PMC 5495223. PMID 28438998.
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