Pasteur effect
The Pasteur effect describes how available oxygen inhibits ethanol fermentation, driving yeast to switch toward aerobic respiration for increased generation of the energy carrier adenosine triphosphate (ATP).[1] More generally, in the medical literature, the Pasteur effect refers to how the cellular presence of oxygen causes in cells a decrease in the rate of glycolysis and also a suppression of lactate accumulation. The effect occurs in animal tissues, as well as in microorganisms belonging to the fungal kingdom.[2][3]
Discovery
[edit]The effect was described by Louis Pasteur in 1857 in experiments showing that aeration of yeasted broth causes cell growth to increase while the fermentation rate decreases, based on lowered ethanol production.[4][5]
Explanation
[edit]Yeast fungi, being facultative anaerobes, can either produce energy through ethanol fermentation or aerobic respiration. When the O2 concentration is low, the two pyruvate molecules formed through glycolysis are each fermented into ethanol and carbon dioxide. While only 2 ATP are produced per glucose, this method is utilized under anaerobic conditions because it oxidizes the electron shuttle NADH into NAD+ for another round of glycolysis and ethanol fermentation.
If the concentration of oxygen increases, pyruvate is instead converted to acetyl CoA, used in the citric acid cycle, and undergoes oxidative phosphorylation. Per glucose, 10 NADH and 2 FADH2 are produced in cellular respiration for a significant amount of proton pumping to produce a proton gradient utilized by ATP Synthase. While the exact ATP output ranges based on considerations like the overall electrochemical gradient, aerobic respiration produces far more ATP than the anaerobic process of ethanol fermentation. The increased ATP and citrate from aerobic respiration allosterically inhibit the glycolysis enzyme phosphofructokinase 1 because less pyruvate is needed to produce the same amount of ATP.
Despite this energetic incentive, Rosario Lagunas has shown that yeast continue to partially ferment available glucose into ethanol for many reasons.[1] First, glucose metabolism is faster through ethanol fermentation because it involves fewer enzymes and limits all reactions to the cytoplasm. Second, ethanol has bactericidal activity by causing damage to the cell membrane and protein denaturing, allowing yeast fungus to outcompete environmental bacteria for resources.[6] Third, partial fermentation may be a defense mechanism against environmental competitors depleting all oxygen faster than the yeast's regulatory systems could fully switch from aerobic respiration to ethanol fermentation.
Practical implications
[edit]The fermentation processes used in alcohol production is commonly maintained in low oxygen conditions, under a blanket of carbon dioxide, while growing yeast for biomass involves aerating the broth for maximized energy production. Despite the bactericidal effects of ethanol, acidifying effects of fermentation, and low oxygen conditions of industrial alcohol production, bacteria that undergo lactic acid fermentation can contaminate such facilities because lactic acid has a low pKa of 3.86 to avoid decoupling the pH membrane gradient that supports regulated transport.[7]
See also
[edit]- Ethanol fermentation
- Fermentation (biochemistry)
- Facultative anaerobic organism
- Allosteric regulation
References
[edit]- ^ a b Lagunas R (1981-01-01). "Is Saccharomyces cerevisiae a typical facultative anaerobe?". Trends in Biochemical Sciences. 6: 201–203. doi:10.1016/0968-0004(81)90073-6. ISSN 0968-0004.
- ^ "Pasteur effect". The Free Dictionary by Farlex.
- ^ Dang, Chi Van; Kim, Jung-Whan (2018). "Convergence of Cancer Metabolism and Immunity: An Overview". Biomolecules & Therapeutics. 26 (1): 4–9. doi:10.4062/biomolther.2017.194. PMC 5746032. PMID 29212301.
- ^ Pasteur L (1857). "Mémoire sur la fermentation applée lactique" [Dissertation on apple lactic fermentation]. Comptes rendus de l'Académie des Sciences (in French). 45 (913–916): 1032–1036.
Pasteur, L. (1995). "Mémoire sur la fermentation appelée lactique (Extrait par l'auteur)". Molecular Medicine. 1 (6): 599–601. PMC 2229983.
Brock, Thomas D. (1995). "Report on the Lactic Acid Fermentation (Author's abstract)". Molecular Medicine. 1 (6): 601–602. doi:10.1007/BF03401600. S2CID 92130442. - ^ Vadlakonda L, Dash A, Pasupuleti M, Anil Kumar K, Reddanna P (2013). "Did we get Pasteur, Warburg, and Crabtree on a right note?". Frontiers in Oncology. 3: 186. doi:10.3389/fonc.2013.00186. PMC 3710993. PMID 23875175.
- ^ McDonnell G, Russell AD (January 1999). "Antiseptics and disinfectants: activity, action, and resistance". Clinical Microbiology Reviews. 12 (1): 147–179. doi:10.1128/CMR.12.1.147. PMC 88911. PMID 9880479.
- ^ Beckner M, Ivey ML, Phister TG (October 2011). "Microbial contamination of fuel ethanol fermentations". Letters in Applied Microbiology. 53 (4): 387–394. doi:10.1111/j.1472-765X.2011.03124.x. PMID 21770989. S2CID 6941273.