Taxane 10beta-hydroxylase

taxane 10-beta-hydroxylase
Identifiers
EC no.	1.14.13.76
CAS no.	337514-75-7
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a taxane 10beta-hydroxylase (EC 1.14.13.76) is an enzyme that catalyzes the chemical reaction

taxa-4(20),11-dien-5alpha-yl acetate + NADPH + H⁺ + O₂ $\rightleftharpoons$ 10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + NADP⁺ + H₂O

The 4 substrates of this enzyme are taxa-4(20),11-dien-5alpha-yl acetate, NADPH, H⁺, and O₂, whereas its 3 products are 10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is taxa-4(20),11-dien-5alpha-yl acetate,NADPH:oxygen oxidoreductase (10beta-hydroxylating). This enzyme participates in diterpenoid biosynthesis.

References

• Wheeler AL, Long RM, Ketchum RE, Rithner CD, Williams RM, Croteau R (2001). "Taxol biosynthesis: differential transformations of taxadien-5 alpha-ol and its acetate ester by cytochrome P450 hydroxylases from Taxus suspension cells". Arch. Biochem. Biophys. 390 (2): 265–78. doi:10.1006/abbi.2001.2377. PMID 11396929.
• Jennewein S, Rithner CD, Williams RM, Croteau RB (2001). "Taxol biosynthesis: taxane 13 alpha-hydroxylase is a cytochrome P450-dependent monooxygenase". Proc. Natl. Acad. Sci. U.S.A. 98 (24): 13595–600. Bibcode:2001PNAS...9813595J. doi:10.1073/pnas.251539398. PMC 61086. PMID 11707604.